![Fig. 3. Shear stress inhibits the gene expression of ICAM-1, VCAM-1, and E-selectin in ECs induced by coculture with SMCs. / ECs cultured alone (EC/φ) or cocultured with identical ECs (EC/EC) or SMCs (EC/SMC) were maintained in a static condition or exposed to a shear stress of 12 dyne/cm2 for 6 hours and their mRNA levels were determined by RT-PCR analysis, as described in “Materials and methods.” Amplification of cDNA was performed in parallel samples using human GAPDH primers. Data are presented for expression of ICAM-1 (A), VCAM-1 (B), and E-selectin (C) as a percentage change in band density normalized to GAPDH samples (ie, differences from densities in static control ECs cultured alone [Control EC/φ]), and are shown as mean ± SEM from 4 independent experiments. *P < .05 vs control EC/φ.#P < .05 vs control EC/SMC.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/7/10.1182_blood-2002-08-2560/3/h80734041003.jpeg?Expires=1723292110&Signature=F7dtmfGukSvwXiA~Xrp7p0lR1fLeJ9plEgEsprL2~sqpDIG3O4Pjx-SN6X8tgCvDfRceoZTxyqM4W1Px6SrFPqX0TU9g0rQHhw1CQO15uEt2Z7nBhU-DDLt1pcl~XEZPSI-uabCTgjgioY60o5f~a2pp9FzqKOVBv0OZd0n1xzZPRCwNzqax-qvryLAMosxZWpAw91P9yEsq21asjLd-mI97UYWlRAE6HhbX3pt7VH5JBplsiQGvmLUjNXOVGja0C03ORhok4-AGKFBGs0cU0Rjl0ES5hS0CFOwrUXvyc5-6rckblX85Zni21l8jR8oufnp0lgMxHOcWTIsNAWtXIA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Shear stress inhibits the gene expression of ICAM-1, VCAM-1, and E-selectin in ECs induced by coculture with SMCs.
ECs cultured alone (EC/φ) or cocultured with identical ECs (EC/EC) or SMCs (EC/SMC) were maintained in a static condition or exposed to a shear stress of 12 dyne/cm2 for 6 hours and their mRNA levels were determined by RT-PCR analysis, as described in “Materials and methods.” Amplification of cDNA was performed in parallel samples using human GAPDH primers. Data are presented for expression of ICAM-1 (A), VCAM-1 (B), and E-selectin (C) as a percentage change in band density normalized to GAPDH samples (ie, differences from densities in static control ECs cultured alone [Control EC/φ]), and are shown as mean ± SEM from 4 independent experiments. *P < .05 vs control EC/φ.#P < .05 vs control EC/SMC.
