IL-17 elicits neovascularization in rat cornea assay, and the addition of anti–IL-17 mAb into Matrigel suppresses the neovessel formation induced by CD4 T cells in mouse Matrigel assay.

(A) No angiogenic response to PBS was observed. (B-D) Vigorous angiogenic response to mouse IL-17 or human bFGF was observed. (E) IL-17–induced vessel formation (250 ng) was not abolished by control mAb. (F) IL-17–induced vessel formation (250 ng) was completely abolished by neutralizing anti–IL-17 mAb. (G) Matrigel plug containing CD4 T-cell–conditioned media plus 20 μg/mL control Ab. (H) Matrigel plug containing CD4 T-cell–conditioned media plus 20 μg/mL anti–IL-17 mAb. (I) Conditioned media from CD4 T cells were shown to induce neovascularization (T cells versus control;P < .05). The addition of neutralizing anti–IL-17 mAb to Matrigel resulted in marked reduction in neovessel formation elicited by CD4 T-cell–conditioned media (T cells or T cells + control Ab versus T cells + anti–IL-17 mAb; *P < .05). Results are expressed as percentage ± SD of the vessel area to the total Matrigel area assessed from 3 stained sections per Matrigel plug. Each individual experimental group included 5 mice. The result is a representative of 2 independent experiments.