Ten-color flow-cytometric analysis of CD57⁺CD8⁺T cells.

PBMCs from a healthy volunteer were stimulated with anti-CD3 in the presence of BFA for 5 hours. Following stimulation, the cells were surface stained with anti–CD3 Texas red PE (TRPE), anti–CD8 Cy7PE, anti–CD27 APC, anti–CD28 PE, anti–CD45RO Cy7APC, anti–CD45RA FITC, anti–CD57 Alx430, and anti–CD62L Cy5.5PE followed by intracellular staining for anti–IFN-γ Alx594. Plots were gated through CD3 and CD8 followed by additional gating for CD45RA and CD27 or for CD57 then CD45RO or CD45RA as shown by arrows. Consistent patterns of coexpression of these molecules were observed in 6 healthy individuals (Table 3).