Quantification of B7-H1–specific mRNA.

(Upper panels) Quantification of B7-H1–specific mRNA in resting (unstimulated; left panel) or in anti-CD3⁺anti-CD28⁻–stimulated (right panel) PBMCs of 3 representative healthy controls. (Lower panels) Quantification of B7-H1–specific mRNA in resting (unstimulated; left panel) or in anti-CD3⁺anti-CD28–stimulated (right panel) PBMCs of 3 representative HIV-infected patients. The upper bands show cDNA retrotranscribed from mRNA extracted from PBMCs; the lower bands show different dilutions (eg, 500, 250, 100) of the specific competitors. The arrows show the dilution at which equivalence is observed between sample cDNA and the specific competitors.