Fig. 6.
![Fig. 6. US2 targets HFE for proteasome-mediated degradation. / 293/HFE and 293/HFE/US2 cells were untreated or treated with the proteasome inhibitor MG132 followed by metabolic labeling with35[S]methionine for 30 minutes, chase for 50 minutes, and immunoprecipitation. Cell lysates were treated with N-glycanase (0.2 units) as indicated, and immunoprecipitated with mAb 2F5 (anti-HFE/β2m, A) followed by anti-HFE CT (anti-HFE heavy chains, B). Immunoprecipitates were separated on 8% SDS-PAGE. Short (A,B) and long exposure of the boxed area (C) are presented. * indicates a nonspecific band.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/7/10.1182_blood-2002-07-2158/3/h80734045006.jpeg?Expires=1724148198&Signature=e0FvZjQq4Odhxx84S~AOvNo2dKnQks1HsdkniOr~lYGhwPErJJLrk57nUUuRg5PNDXXF7Ce02kKUtDhAzB3ig2Jst15oxcTpjeRPpMmILF8w7f-ludYsiaZFShDkLGGFi8X7RzdP~F8Y~4ot-geDVhLp~tIGJ~Vfugbe1NMxATWdsql2ZgrWLvk15qA0B0710hvOueCQt5MtC1ifCw6Q3fV9F9UF2I7Wb7ZgynGRVLAd7qya7Y3EhIsbLXFC7vP81UMW2Pj79tm7YrWSO3X3kDXhCQdNtJbCQyAXY77nufPwCvWjBS9h~HfMG5aebWXT2G4~sF0pAacFI8R9am9a6g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
US2 targets HFE for proteasome-mediated degradation.
293/HFE and 293/HFE/US2 cells were untreated or treated with the proteasome inhibitor MG132 followed by metabolic labeling with35[S]methionine for 30 minutes, chase for 50 minutes, and immunoprecipitation. Cell lysates were treated with N-glycanase (0.2 units) as indicated, and immunoprecipitated with mAb 2F5 (anti-HFE/β2m, A) followed by anti-HFE CT (anti-HFE heavy chains, B). Immunoprecipitates were separated on 8% SDS-PAGE. Short (A,B) and long exposure of the boxed area (C) are presented. * indicates a nonspecific band.
