Fig. 4.
![Fig. 4. US2 expression leads to decreased cell surface levels of HFE and HLA class I molecules in 293/HFE/US2 and 293/US2 cells, respectively. / The 293/HFE/US2 (clones 1-3) cells were analyzed by FACS (A) with anti-HFE mAb 2F5 and anti-HLA mAb W6/32. 293/US2 (clones 1, 2) cells were analyzed by FACS (B) with anti-HLA mAb W6/32, metabolically labeled with 35[S]methionine for 1 hour, followed by immunoprecipitation with Abs directed against free HLA class I HC (C, top), and analyzed for expression of total HLA class I (C, bottom) by Western blot hybridization of fractionated cell lysates with Abs directed against free HLA class I HC. Bold-lined histograms indicate staining with first Ab; regular-lined histograms, background staining.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/7/10.1182_blood-2002-07-2158/3/h80734045004.jpeg?Expires=1724148762&Signature=qwGYW67QySo5LLIxZGhe86Y6G37H6JHbdkb1Uw2evZTpXlI~6e~txkmmFufsuZcrcNrui1jnZpFJNiO3y3IVHrq1W5aRLuHqQSC5KlLjg7Ud9c1j1KX04WSPFg7Y77FnTpbXHm~u~A-wiMqHRCZznxQ9ZvdDue8fLgzOAZAk4v3~79U-zFKldPFOe8pGH-WGNU4mRE7IEYpkc7O~EYnx7TtiB~jMY3cLfSHjcTDdtYrLPQVRL5~mBJdeaqdW7qNWmg8897PkGKQzBFQSvN2Nn0FpuFM9LQV0whlhIi~b13TTrrJw4iKQoZsO3H6FWlqZZXa0bwYh5WQgukB3dGA3Cg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
US2 expression leads to decreased cell surface levels of HFE and HLA class I molecules in 293/HFE/US2 and 293/US2 cells, respectively.
The 293/HFE/US2 (clones 1-3) cells were analyzed by FACS (A) with anti-HFE mAb 2F5 and anti-HLA mAb W6/32. 293/US2 (clones 1, 2) cells were analyzed by FACS (B) with anti-HLA mAb W6/32, metabolically labeled with 35[S]methionine for 1 hour, followed by immunoprecipitation with Abs directed against free HLA class I HC (C, top), and analyzed for expression of total HLA class I (C, bottom) by Western blot hybridization of fractionated cell lysates with Abs directed against free HLA class I HC. Bold-lined histograms indicate staining with first Ab; regular-lined histograms, background staining.
