Characterization of HEK 293 cells transfected with HFE and US2.

HEK 293, 293/HFE, and US2-transfected 293/HFE clones (1-3) were analyzed for expression of US2, HFE, and actin mRNA by RT PCR (A) and US2 protein by metabolic labeling and immunoprecipitation with rabbit anti-US2 (αUS2) Abs (B).