Fig. 2.
![Fig. 2. HFE-transfected HEK 293 cells demonstrate induced synthesis of TfR and reduced synthesis of ferritin. / HEK 293 and 293/HFE cells (clones 1-5) were metabolically labeled with35[S]methionine for 1 hour, and the cell lysates were immunoprecipitated with Abs directed against HFE/β2m, 2F5, and ferritin. Cell lysates were fractionated and tested by Western blot analysis for the expression of TfR with H68.4 Ab.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/7/10.1182_blood-2002-07-2158/3/h80734045002.jpeg?Expires=1722765263&Signature=fuLgqPOOb7pqQvn9W0v3TR5Ssmz7P-O2l4J7YYG2-JvtFsZ0TzWyYn7IyS0JgCLGEqKf0st4Goi~bnd3zM7Cimvai160-Xtbohxw9Th8zFEjmz2YVr~xQDX2UwxuyyVGx-g9QybPe8peM8ZBhNdMCYaXVliOBZusaAqVP0ZiauxYNKMXgERVLPTrp08O4YxP--45afEPTwjzbA1~JIZbbMNjoKqlsh4RmlKcBv7BMG~86GRIuDRea1dRFv3EPfFYdTEcH5ai83LTLYFphDANLxf75iH1cLfRv9~cfuRr1Vf5IZ8uJgprvfLgJpjNtCfuMC7EnwLXEUvt9VrVZfwMsw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HFE-transfected HEK 293 cells demonstrate induced synthesis of TfR and reduced synthesis of ferritin.
HEK 293 and 293/HFE cells (clones 1-5) were metabolically labeled with35[S]methionine for 1 hour, and the cell lysates were immunoprecipitated with Abs directed against HFE/β2m, 2F5, and ferritin. Cell lysates were fractionated and tested by Western blot analysis for the expression of TfR with H68.4 Ab.
