Fig. 1.
![Fig. 1. Cell surface and intracellular expression of HFE and class I MHC molecules in HFE-transfected HEK 293 cells. / HEK 293 and HFE-transfected HEK 293 cells were analyzed by FACS for cell surface expression of HFE and HLA (A) or metabolically labeled with 35[S]methionine for 1 hour and lysed, and cell lysates were immunoprecipitated (B) with Abs directed against hHFE (2F5) HLA (W6/32), and HLA heavy chains (αHC). Abs were used in excess as indicated in “Materials and methods.” Bold-lined histograms indicate staining with the first Ab; regular-lined histograms, background staining.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/7/10.1182_blood-2002-07-2158/3/h80734045001.jpeg?Expires=1722765296&Signature=pCvX7kJs3ueMxgCRCn1WQAlPhpSBKeyVfaoeQ27NdU2qDZ2Eu1tBK1WsqroEPIUQSOKg4GOAyfIe4zzIFoe0hwyyVFfscTH5RZTsWZbH6eJ6K37CHNHH8InEY0fKVGm8ScVeFgzxKUOS8PMGrSBx4MOreLqX-YngZev7LZzqL3OFQJ6C3T0GXaijXN6snV7NWyiNJ-n3x~CzPxQshVoWFNYUKJV1gV6BlZByuDvIEPtZPeH~S3LyIYZTZ1tabtnFvYPS~vE5VE0CZV4UrpnCgaCNlOPYrl-n3SxDl-R9Ns15Byg17RjAALPiZj68pCQt-2hMSKx9LLZw52z2eHwIAg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cell surface and intracellular expression of HFE and class I MHC molecules in HFE-transfected HEK 293 cells.
HEK 293 and HFE-transfected HEK 293 cells were analyzed by FACS for cell surface expression of HFE and HLA (A) or metabolically labeled with 35[S]methionine for 1 hour and lysed, and cell lysates were immunoprecipitated (B) with Abs directed against hHFE (2F5) HLA (W6/32), and HLA heavy chains (αHC). Abs were used in excess as indicated in “Materials and methods.” Bold-lined histograms indicate staining with the first Ab; regular-lined histograms, background staining.
