Effects of PI3K and ERK inhibitors on CD40-induced MM.1S migration.

A transmigration assay was performed as described in “Materials and methods.” Serum-starved MM.1S cells, with and without pretreatment with inhibitors for 1 hour, were seeded in the upper chamber in the transwell cluster plate. At 6 hours after CD40 activation by G28.5 anti-CD40 mAb (5 μg/mL) in lower chamber, migrating cells in the lower chamber were collected and counted. Data are the means ± SD of triplicate determinations.