Fig. 3.
Phagocytosis of fluorochrome-labeled polystyrene beads by PMNs.
Purified PMNs (2 × 105) were incubated with the indicated stimuli in the presence of 5 × 106fluorochrome-labeled microspheres for 45 minutes at 37°C. Cells were washed and fixed in 1% paraformaldehyde. Fluorescence intensity was evaluated by FACS. The results shown are representative of 4 independent experiments with different donors. Statistical analysis of all experiments showed a significant increase in mean fluorescence with PMA, LPS, or Gp96 compared with the medium control (n = 4;P < .05 by Student t test).
