Fig. 4.
SU11657 inhibits activated FLT3.
MV 4;11 cells were incubated with SU11657 at the indicated concentration for 2 hours. Lysates were prepared and immunoprecipitated with an anti-FLT3 antibody. After SDS-PAGE and transfer to nitrocellulose, blots were probed with an antiphosphotyrosine antibody (top) and subsequently stripped and reprobed with an anti-FLT3 antibody (bottom). The IC50 for inhibition of FLT3-ITD phosphorylation is approximately 50 nM.
