Fig. 2.
Fig. 2. Study of apoptosis in purified CD33+ and CD15+ cells. / (A) Scattergram of anti-CD15 fluorescence versus right-angle side scatter (SSC) of purified CD33+ cells allows distinction between CD33+/CD15+ (R2) and CD33+/CD15− (R3) cells. (B) Scattergram of anti-CD15 versus anti-Fas fluorescence gated on R2 showing Fas+ (R4) and Fas− (R5) CD33+/CD15+ cells. (C) Scattergram of anti-CD15 versus anti-Fas fluorescence gated on R3 showing Fas+ (R4) and Fas− (R5) CD33+/CD15− cells. (D) Scattergram of forward scatter (FSC) versus 7-AAD fluorescence gated on R2 showing apoptotic and live CD33+/CD15+ cells. (E) Scattergram of anti-CD15 fluorescence versus SSC of purified CD33−/CD15+ cells (R6). (F) Scattergram of FSC versus 7-AAD fluorescence gated on R6 showing apoptotic and live CD33−/CD15+ cells.

Study of apoptosis in purified CD33+ and CD15+ cells.

(A) Scattergram of anti-CD15 fluorescence versus right-angle side scatter (SSC) of purified CD33+ cells allows distinction between CD33+/CD15+ (R2) and CD33+/CD15 (R3) cells. (B) Scattergram of anti-CD15 versus anti-Fas fluorescence gated on R2 showing Fas+ (R4) and Fas (R5) CD33+/CD15+ cells. (C) Scattergram of anti-CD15 versus anti-Fas fluorescence gated on R3 showing Fas+ (R4) and Fas (R5) CD33+/CD15 cells. (D) Scattergram of forward scatter (FSC) versus 7-AAD fluorescence gated on R2 showing apoptotic and live CD33+/CD15+ cells. (E) Scattergram of anti-CD15 fluorescence versus SSC of purified CD33/CD15+ cells (R6). (F) Scattergram of FSC versus 7-AAD fluorescence gated on R6 showing apoptotic and live CD33/CD15+ cells.

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