Effect of rPSGL-Ig pretreatment on thioglycollate-induced peritonitis.

Mice were pretreated (15 minutes) with either saline, CD4-Ig (1 mg/kg, subcutaneously), rPSGL-Ig (1 mg/kg, subcutaneously), RB40.34 (10 μg, subcutaneously), or 10E6 (10 μg, subcutaneously), followed 15 minutes later by 1 mL intraperitoneal injection of thioglycollate (▪) or saline (■). Peritoneal lavage was performed 4 hours after stimulation, and neutrophil numbers were determined. The data represent the means ± SEMs of 7 mice in vehicle, CD4-Ig–, and rPSGL-Ig–pretreated groups, and 8 mice in all other groups. Significant difference from vehicle control is indicated by **P < .01.