Northern blot analysis of Felic.

Hematopoietic and nonhematopoietic tissues were analyzed by Northern blotting with a randomly labeled probe of Felic. (A) Total RNA of multiple normal human tissues was evaluated: PBL indicates peripheral blood lymphocytes; C, Colon; SI, small intestine; Ov, ovary; Te, testis; Pr, prostate; Th, thymus; and Sp, spleen. Two transcripts are present, except for T cells (thymus and peripheral blood lymphocytes). (B) Total RNA was harvested from myeloid cell lines Mo7e, HL60, TF-1, and 32Dcl3 and lymphoid cell lines Molt and Jurkat, which were analyzed. Comparable amounts of RNA were loaded, as verified by ethidium bromide staining (data not shown).