Expression of SgIGSF and intercellular adhesion molecules in CMCs derived from MITF mutant mice.

(A) Expression of SgIGSF mRNA in B6^+/+, B6^mi/mi, and B6^tg/tgCMCs. RNA (10 μg of total RNA) from CMCs of 3 genotypes was electrophoresed and hybridized with the clone 236 probe. After stripping, the blot was hybridized with the β-actin probe to indicate the amount of RNA loaded per lane. (B) Expression of the SgIGSF protein and intercellular adhesion molecules in B6^+/+, B6^mi/mi, B6^tg/tg, and B6^Miwh/MiwhCMCs. Lysates of the indicated cells were electrophoresed and blotted with antibodies against SgIGSF, E-cadherin, N-cadherin, ICAM-1, and integrin β3. (C) Western blot analysis of NIH/3T3 cells and those transfected with SgIGSF cDNA (NIH/3T3^SgIGSF). The lysates of the indicated cells were electrophoresed and blotted with the SgIGSF antibody and then probed again with the anti-α-tubulin antibody to indicate the total amount of proteins loaded per lane. The molecular weight scale is shown to the right of the blot.