Fig. 5.
Southern blot analysis.
Genomic DNA extracted from 5 PMBLs, 5 NM-DLBLs, and the MedB-1 and Ramos B-cell lines were subjected to EcoRI digestion, agarose gel electrophoresis, nylon N+ membrane transfer, and hybridization with an α-32P–labeled PCR-derivedFIG1 cDNA probe. A schematic map of the FIG1 gene is represented at the top of the figure (not drawn to scale). The probe used (bold line) spans FIG1 exons 5, 6, and 7, and hybridized with 10-kb and 4.5-kb DNA fragments in all samples.
