Nur77 and PAI-1 expression in normal and atherosclerotic vascular tissues.

(A-D) Minimal expression for Nur77 (A) and PAI-1 (B) in normal vessels; high expression of both proteins in the plaque area in endothelial cells and smooth muscle cells in an atherosclerotic coronary artery (C-D). The 50-μm scale bar in panel D applies to panels A-D. (E-G) Coimmunostaining of Nur77 and PAI-1 in a human atherosclerotic lesion. The vessel was stained for Nur77 (green, E), PAI-1 (red, F), or CD31 (blue, G). The 100-μm scale bar in panel G applies to panels E-G. (H-J) Endothelial cells of vasa vasora in human atherosclerotic coronary arteries express both Nur77 (H) and PAI-1 (J), which colocalize (I). The 20-μm scale bar in panel J applies to panels H-J. (K-M) Expression of Nur77 in a human atherosclerotic vessel (K) with higher expression in the neointima (L) and lower expression in the media (M). Panels L and M show further magnification of the indicated areas of panel K.