TCRαδ, TCRγ, and TCRβ loci and TCRδ and TCRβ profiles in T-ALL.

(A) Schematic representation (not to scale) of the TCRαδ, TCRγ, and TCRβ loci, after the IMGT (International ImMunoGeneTics) database http://imgt.cines.fr (initiator and coordinator, Marie-Paule le Franc, Montpellier, France).66 Orientation of the PCR primers are shown as arrows. (B) Representative Southern blot patterns obtained with a Jδ1 probe (top) and TCRδ heteroduplex PCR (bottom). GL indicates gemline; R, rearranged; Del, deleted. (C) Representative TCRβ PCR profiles. The immunophenotypic group is indicated for each patient, and the type of rearrangement is shown. TCRβ PCR detects V(D)J and DJ rearrangements.