Fig. 4.
Demonstration of the FLT3 ITD in AML blasts.
Total RNA was extracted from 1 × 106 to 5 × 106 blast cells. PCR was performed on total cDNA with specific primers flanking exon 11 and 12 of the FLT3gene where the ITD usually occurs. *The positive control (+ve) was an AML sample previously shown to contain this mutation, and the negative control (-ve) was cDNA from cells from a patient with acute lymphoblastic leukemia that did not show the Flt-3 ITD.
