Tolerance for human apo A-I after transfer withAdAT4.
(A) Human apo A-I expression after gene transfer with 5 × 108 pfu AdAT4 (▪) in Balb/c mice, which were subcutaneously immunized with human apo A-I 3 weeks after gene transfer and rechallenged with subcutaneous human apo A-I immunization 6 weeks after gene transfer. (B) Inverse of the titer of antibodies against human apo A-I after subcutaneous immunization with human apo A-I protein in naı̈ve Balb/c mice (▵) and after gene transfer with 5 × 108 pfu AdAT4 in Balb/c mice, which were subcutaneously immunized with human apo A-I 3 weeks after gene transfer and rechallenged with subcutaneous human apo A-I immunization 6 weeks after gene transfer (●). Arrows indicate the time point of subcutaneous immunization in mice treated withAdAT4.
