FTI277 and Ras N17 inhibit GM-CSF– but not

C albicans–induced MAPK/ERK activation. Freshly isolated PMNs, pretreated with DMSO or with 15 μM FTI277 or infected with N17Ras, were stimulated with GM-CSF (100 U/mL) (A) or C albicans (B) for 0 to 5 minutes at 37°C. Cell lysates were immunoblotted with antiactive MAPK (upper panel) and reprobed with anti-panMAPK/ERK (lower panel) for equal loading. Results shown are representative of 3 separate experiments.