C albicans –induced ERK activation in PMNs is Ras independent.

(A) Freshly isolated PMNs were treated with medium alone, DMSO, or various doses of the farnesyl-transferase inhibitor, FTI277, for 24 hours prior to the addition of C albicans for 0 to 5 minutes at 37°C. Cell lysates were immunoblotted with antiactive MAPK (upper panel) and then stripped and reprobed with anti-panERK for equal loading (lower panel). (B) The same aliquots of PMNs were also tested for their ability to inhibit C albicans growth. Results shown are representative of 4 separate experiments.