Fig. 2.
Effect of PD098059 on PMN function.
(A) PMNs were pretreated with medium, DMSO, or increasing doses of PD098059 for 2 hours at 37°C prior to testing for their ability to inhibit the growth of C albicans using3H-glucose incorporation. Results shown are representative of at least 3 separate experiments. (B) PMNs, pretreated with various doses of PD098059 for 2 hours at 37°C, were mixed withC albicans for 0 to 5 minutes at 37°C. Cell lysates were then analyzed by immunoblotting with antiactive MAPK (upper panel) or anti-panERK to check for equal loading (lower panel). Results shown are representative of 5 separate experiments.
