Induction of MAPK/ERK activation by GM-CSF and

C albicans. (A) Freshly isolated PMNs were pretreated with heat-killed C albicans at a PMN/C albicansratio of 1:10 for 0 to 30 minutes at 5 minutes at 37°C, and cell lysates were analyzed by immunoblotting with antiactive MAPK (upper panel) and then stripped and reprobed with anti-panERK to check for equal loading (lower panel). (B) PMNs, pretreated with GM-CSF (100 U/mL) at 37°C for 0 to 30 minutes, were lysed and sequentially probed for active-MAPK and anti-panERK. Results shown are representative of 3 separate experiments.