Activation of AKT kinase by plasma or HA.

Activation of AKT was determined as described in “Materials and methods.” Cells from 2 different patients were used in A and B. Serum, delipidated serum (dSerum), plasma, and FT were added as 50% mixtures with basal medium except where indicated. HA was at 25 mg/mL. Data are representative of 8 experiments.