Albumin depletion compromises the antiapoptotic action of plasma.

(A) SDS-polyacrylamide gel electrophoresis of plasma. FT indicates flow-through from Cibacron Blue-Sepharose column; and FT + HA, flow-through supplemented with 50 mg/mL HA. (B) Percentage of apoptotic cells and (C) loss of viability of untreated CLL cells or cells pretreated with 80 μM chlorambucil and incubated for 24 hours with the indicated supplements. The data are representative of 3 experiments.