Primary colony formation by gcsfr^−/−bone marrow progenitors transduced with different G-CSF-R constructs.

Colonies were grown in the presence of 100 ng/mL G-CSF. Left panel shows mean colony numbers ± SD per 1000 infected bone marrow cells from triplicate colony dishes; data are representative of 4 independent experiments. Colony numbers were normalized to the numbers of infected cells based on puromycin resistance of CFU-GMs (responsive to GM-CSF) to correct for differences due to variations in transduction efficiencies. Right panel shows mean numbers of cells per colony ± SD from triplicate dishes.