Expression of G-CSF-R mutants.

(A) Schematic representation of cytoplasmic domains of human G-CSF-R wild-type and tyrosine substitution mutants showing positioning of conserved tyrosines relative to membrane proximal Box1 and Box2. (B) Flow cytometric analysis of G-CSF-R expression ongcsfr^−/− bone marrow cells retrovirally transduced with G-CSF-R WT and tyrosine mutants shown in panel A. Bold histograms indicate cells stained with biotinylated G-CSF-R antibodies and SA-PE; dotted histograms, cells stained with SA-PE only.