Expression analysis of cytoglobin.

(A) RNA was isolated from the indicated organs derived from adult mice, and expression of cytoglobin was determined by RT-PCR. The number of PCR cycles was within the linear range of the reaction (not shown). (B) To quantitate the amplicons, the bands were scanned on a Molecular Dynamics Typhoon instrument (Sunnyvale, CA), and the relative expression levels of cytoglobin were calculated using cyclophilin A as a reference.