Dexamethasone-induced apoptosis of 5T2MM cells during disease progression.

5T2MM cells from different stages of disease progression were sorted as described. The MM cells were incubated with or without BMSCs and 5 μM dexamethasone was added where indicated. Apoptosis was analyzed by staining of the cells with annexin V and propidium iodide after overnight incubation. Data represent mean values ± SD of 3 mice (3 independent experiments) at each disease stage.