Evolution of serum paraprotein concentration during disease progression and correlation between serum paraprotein concentration and 5T2MM myeloma tumor load in bone marrow.

(A) Evolution of serum paraprotein concentration during disease progression. Mice injected with 5T2MM cells were bled before being killed at indicated time points. Paraprotein in serum was quantified by protein electrophoresis. Each point represents mean value ± SD of 3 animals. (B) Correlation between serum paraprotein concentration and 5T2MM myeloma tumor load in the bone marrow. Paraprotein concentrations were measured as described in Figure 2A legend. Bone marrow mononuclear cells from the same mice were analyzed for 5T2MM tumor load by flow-cytometric staining with anti-idiotype–specific monoclonal antibodies. Each point represents mean value of 3 mice ± SD.