Percentages of G6⁺ B cells in the peripheral blood.

(A) Peripheral blood was stained with mouse MoAb G6 followed by sheep antimouse-FITC and subsequently with CD5-PE/CD19-RPE-Cy5. On an initial 10 000-cell acquisition, the B cells were defined as shown by the gates R1 (lymphocytes) and R2 (CD19⁺ lymphocytes). Approximately 900 000 total events per tube were acquired in an R1-plus-R2 live gate to give percentages for G6 reactivity. (B) Comparison of the percentage of G6⁺ lymphocytes in 35 young (▪) and 42 (▴) elderly individuals.