Fig. 2.
![Fig. 2. Expression of BCL3 mRNA and Bcl-3 protein in ALCL, HD, and other hematologic tumor cell lines. / (A) Northern blotting analysis of RNA extracted from ALCL, HD, and other hematologic tumor cell lines. Total RNA samples (10 μg) were electrophoresed on a MOPS (3-[N-Morpholino]propanesulphonic acid)–formaldehyde gel, and the patterns of the 28S and 18S ribosomal RNA bands were observed to verify the comparable amounts of RNA in each lane (bottom). (B) Western blotting analysis of ALCL, HD, and other hematologic tumor cell lines for Bcl-3 protein expression. Cell lysates were prepared from 1 × 106 cells, and an aliquot was loaded. Bcl-3 migrates around 58 kDa in sodium dodecyl sulfate (SDS)–polyacrylamide gels.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/7/10.1182_blood-2002-08-2464/3/h80734087002.jpeg?Expires=1724970587&Signature=um8zvHy13WbP6mTO51~PPuRxKlcC16saRCDZMlAsAZ~aYAO6YwAkk49DsJ32l1CcxrIfipAaIK5UzDasJtgFM-Ynlxjhv8JQbnTiiXqYpJz7Lbljjjm2DOOOFl~NAclp6uWjEzYp013Xwlf9u-uX6V6HqZGyDkSZBMets2RTPNwxEdDVfp3XT4UnXdFj~i875Xuh9IiWjGhM--s42s0fnybJZaNyXd8nnjdWUMh71-BOsSASP~5fr4Wk3BegQcSrxSBUCU1ElXI-~q6hIKd5AA2JYin3uK3gYUVljxzCh5Av54I2DfDX~g4J0RYpGEAyfUy9qLklmy1oHgER-wqJ7w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of BCL3 mRNA and Bcl-3 protein in ALCL, HD, and other hematologic tumor cell lines.
(A) Northern blotting analysis of RNA extracted from ALCL, HD, and other hematologic tumor cell lines. Total RNA samples (10 μg) were electrophoresed on a MOPS (3-[N-Morpholino]propanesulphonic acid)–formaldehyde gel, and the patterns of the 28S and 18S ribosomal RNA bands were observed to verify the comparable amounts of RNA in each lane (bottom). (B) Western blotting analysis of ALCL, HD, and other hematologic tumor cell lines for Bcl-3 protein expression. Cell lysates were prepared from 1 × 106 cells, and an aliquot was loaded. Bcl-3 migrates around 58 kDa in sodium dodecyl sulfate (SDS)–polyacrylamide gels.
