Binding of AJvW-2 to wt-A1 and mutants.

Plateau values for the binding of saturating concentrations of AJvW-2 (A) and the conformation-dependent anti-VWF A1 antibodies, poly–anti-VWF, A21H3, and A7C6 (B), to microtiter plate-coated A1 mutants (2 μg/mL), were determined by ELISA. Results are expressed as the mean percentages ± SDs of triplicate determinations (*P < .01), relative to binding to wt-A1. For space considerations, single-letter codes were used for amino acids.