Influence of uPAR on α_Mβ₂-mediated adhesion to uPA.

(A) uPAR (upper panel), α_Mβ₂ (middle panel), or α_Mβ₂/uPAR (lower panel) transfected HEK293 cells were treated with 0.5 U/mL PI-PLC (black bars), 100 μM M25 peptide (white bars), the control-scrambled M25 peptide SCR (striped bars), or untreated (gray bars) for 30 minutes at 37°C, and then allowed to adhere HMW-tc-uPA or its domains for 30 minutes at 37°C. (B) The indicated transfected cells were preincubated with GFD (0.4 μM) for 30 minutes and then seeded onto kringle domain (KD)–coated wells for 30 minutes at 37°C. After 4 washings with PBS, the adherent cells were quantitated. The results are expressed as means ± SEM of quadruplets from 3 independent experiments.