Effect of α_MI domain–blocking reagents on the adhesion of α_Mβ₂ and α_Mβ₂/uPAR-expressing cells to uPA and its domains.

(A) Effect of α_MI domain reagents. The α_Mβ₂ (upper) or α_Mβ₂/uPAR (lower) HEK293 cells were pretreated with NIF (0.1 μM), mAb 44a, or control mAb W6/32 to MHC-I (20 μg/mL) for 30 minutes at 37°C, and then seeded (2 × 10⁵ cells) onto HMW-tc-uPA or its domains. (B) Effect of α_MI domain. Wells, coated with HMW-tc-uPA or its derivatives, were preincubated with or without recombinant α_MI domain (100 nM) for 1 hour at 37°C, washed once, and the α_Mβ₂/uPAR cells added. (C) Effect of α_MI domain reagents on neutrophil adhesion. The influence of NIF (0.1 μM; white bars) and α_MI domain (100 nM; gray bars), or no treatment (black bars) on the adhesion of PMA-stimulated neutrophils to HMW-tc-uPA or its domains. In each case, adhesion was measured after 30 minutes. The data are means ± SEM of triple measurements from 3 independent experiments.