FLIP ASOs reduce FLIP expression and enhance IFNγ/FasL-induced apoptosis that is not reduced by SCF in the presence of ASOs.

Day −5 ECFCs were incubated at 37°C with or without SCF (30 ng/mL) for 48 hours, and then the resulting day −7 ECFCs were transfected with FLIP ASOs or control NSOs over 12 hours before incubation with IFNγ (400 U/mL) and FasL (50 ng/mL) over 48 hours. (A) Cell lysates were prepared, and immunoblot analyses were performed with anti-FLIP antibody. (B) Apoptosis was determined by TUNEL assay, and the percentages of apoptotic cells were expressed as the mean ± SD from 4 independent experiments. *P < .05, ASO-treated group versus NSO-treated group (without SCF preincubation); **P < .05, ASO-treated group versus NSO-treated group (with SCF preincubation); ***P < .05, NSO-treated group without SCF preincubation versus NSO-treated group with SCF preincubation; ****P = .21, ASO-treated group without SCF preincubation versus ASO-treated group with SCF preincubation.