Caspase-8 and caspase-3 are activated by IFNγ plus FasL in a time-dependent manner.

Day −6 ECFCs were cultured in medium with or without IFNγ (400 U/mL), FasL (50 ng/mL) at 37°C for 24 to 96 hours. Cell protein lysates were prepared, and immunoblot analyses were performed with anticaspase-8 and anticaspase-3 antibodies.