Fig. 7.
Bcl-2 family protein expression in viable and apoptotic subpopulations of B-CLL cells.
Viable and apoptotic cells were identified by using a flow cytometric gating strategy based on changes in forward and side light scatter (A), and a comparison of the Bcl-2 (light gray bars), Mcl-1 (open bars), and Bax protein (dark gray bars) expression in the viable and apoptotic subpopulations of B-CLL cells treated with 1 × 10−8 M EB1089 was made (B). Both Bcl-2 and Mcl-1 were modulated only in apoptotic cells, indicating that changes in their expression is the consequence of apoptosis rather than the cause.
