Effect of T cells and DCs from CTLA4Ig-treated animals on naive MLRs.

Splenocytes from naive LEW.1A rats were cocultured with irradiated LEW.1W APCs and with (A) splenocytes, (B) DCs, or (C) T cells purified from spleens from the same animals harvested on day 10 after transplantation from 2 CTLA4Ig-treated or 2 control LEW.1A rats that received LEW.1W heart transplants. Cells were cocultured at 1:1 cell ratio in the presence of L-NMMA (5 mM) for 3 or 5 days. Results are expressed as delta (allogeneic − spontaneous) cpm ± SD. Results are representative of 3 experiments with a total of 5 animals in each group. Each curve represents the proliferation of cells from a single animal.