Overexpression of A1-a in Tg mice.

Thymocyte (A) or splenocyte (B) RNA (4 μg/lane) from wild-type (−/−) or A1-a Tg (+/−) littermates was analyzed by hybridization of Northern blots with an A1-a cDNA probe. Two independent A1-a Tg lines, 6.3 and 7.1, are shown. P3 indicates RNA (4 μg) from the macrophagelike cell line P388D1, which constitutively expresses levels of A1 mRNA comparable to activated macrophages or T cells43; Tg A1, location of the predicted fully spliced transgenic transcript; En A1, endogenous A1 mRNA.