A1 and Bcl-2 gene expression in activated splenocytes.

(A-B) Splenocytes from wild-type mice were treated with Con A for the indicated times and gene expression was determined by Northern blot analysis normalized to 28S rRNA. Data from 3 similar experiments are pooled. Within each experiment values are relative to the value for unstimulated cells (set to 1). Each point represents the mean ± SE for 3 to 6 mice, except for 9 hours (n = 1) and 16 hours (n = 2). The total number of measurements is 30 for A1 and 25 for Bcl-2. (C) Purified splenic T cells were activated with αCD3 plus αCD28 for the indicated times and analyzed by the RNase protection assay (RPA). A1 expression is normalized to L32.