CD11c⁺, I-A^{b low-high} cells generated from TNF/LTα/LTβ^−/− BM culture possess stimulatory activity in MLR similar to DCs from wt BM culture.

BM cells (1 × 10⁶ cells/mL) from either wt (●) or TNF/LTα/LTβ^−/− (○, ▵, ■) mice were cultured with GM-CSF and IL-4 for 9 days. CD11c⁺, I-A^{b low-high} (▵), and CD11c⁻, I-A^b−(■) cells were sorted from TNF/LTα/LTβ^−/− BM culture. After 30 Gy irradiation, indicated number of generated cells were cocultured with syngeneic (A) or allogeneic (B) LN cells (1 × 10⁶ cells/mL) for 4 days. During the last 18 hours of the culture, [³H]dThd was added in each well. The values are the means ± SD of quadruplicate wells. Data from 1 of 2 representative experiments are shown. *P < .01 compared with TNF/LTα/LTβ^−/− whole and TNF/LTα/LTβ^−/− CD11c⁻ population.