Fig. 3.
![Fig. 3. The stimulatory activity of cells generated from TNF/LTα/LTβ−/− BM culture is significantly reduced compared with that from wt BM culture. / BM cells (1 × 106 cells/mL) from either wt (●) or TNF/LTα/LTβ−/− (○) mice were cultured with GM-CSF and IL-4 for 9 days. Cells were collected on day 9 and subjected to 30 Gy irradiation. Indicated number of generated cells were cocultured with syngeneic (A) or allogeneic (B) LN cells (1 × 106 cells/mL) for 4 days. During the last 18 hours of the culture, [3H]dThd was added in each well. The values are the means ± SD of quadruplicate wells. Data from 1 of 3 representative experiments are shown. *P < .01 compared with TNF/LTα/LTβ−/− BM culture. CPM indicates counts per minute.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/4/10.1182_blood.v101.4.1477/3/h80433854003.jpeg?Expires=1724238372&Signature=i-H-SktgHGnFPRLzIkH97uFKPo-~xirl-7~Drauo4nQYI6awaSQULdV7O1oFe4coXjlskxhOFzqR9TSg43cD13w5xjgecTks0iEAUr7ZC3BeZA-TaGWJqxHs5rE1yLq87KT-I2VBm6Q2xRrUBbtXnf0gOiGl1Kb~Pn2dyP9ol0Fme2Gfye2YL1OAJhBl9-k5DFjFwc82ZW5ucmqF19JYeQ27GXrnhF84TIGU473PFQZEE7U8y2SAedlSBciFGnjmaDaILSD-ToM-4kt2U0BOB3LDLGD4hTHT~Gf~LEXOqmk18DfStdiKBFCOMjuGGxJ2Is3SYN4g1TPpOwsSIG9oeg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The stimulatory activity of cells generated from TNF/LTα/LTβ−/− BM culture is significantly reduced compared with that from wt BM culture.
BM cells (1 × 106 cells/mL) from either wt (●) or TNF/LTα/LTβ−/− (○) mice were cultured with GM-CSF and IL-4 for 9 days. Cells were collected on day 9 and subjected to 30 Gy irradiation. Indicated number of generated cells were cocultured with syngeneic (A) or allogeneic (B) LN cells (1 × 106 cells/mL) for 4 days. During the last 18 hours of the culture, [3H]dThd was added in each well. The values are the means ± SD of quadruplicate wells. Data from 1 of 3 representative experiments are shown. *P < .01 compared with TNF/LTα/LTβ−/− BM culture. CPM indicates counts per minute.
