Function of bcl-x promoter sequences in stable transfection assays (integrated constructs).
Several of the bcl-x/reporter constructs depicted in Figure5 were further modified to contain a neomycin resistance gene. These were transfected into HCD57 cells, and clones of cells bearing integrated plasmids were selected by culturing in G418. Approximately 50 luciferase-positive cell clones were isolated for each plasmid construct. Luciferase expression was measured for each cell clone; each “x” on the graph represents a cell clone. Numbers on the x-axis indicate group numbers. Group 1 clones bear a plasmid withbcl-x promoter sequence from −1 through −197; Group 2 have promoter sequence from −1 through −1217; Group 3 have sequence from −1 through −1804; and Group 4 have sequence from −1 through −2331. Relative luciferase units are standardized on a per 0.5 × 106 cells basis.
