Retroviral integration patterns were detected by LM-PCR.

(A) Three SF1m-transduced HT1080 cell line clones were analyzed. DNA from each clone was used in separate reactions (1, 2, 3) and mixed together in one reaction (1/2/3). The internal band (IB) originates from the 3′ LTR and is identical for all SF1m vector-transduced cells. (B) Five SF1m-transduced chimeric NOD/SCID mouse BMs analyzed with the optimized LM-PCR protocol. Whole chimeric BM DNA was digested withBsmAI. Nested LM-PCR products were analyzed by agarose gel electrophoresis. Numbers to the left of the blots denote fragment size in kilobases (kb). The control mouse received transplants of untransduced (mock-transduced) human cells. (C) Flanking sequences of clones isolated from mouse E3M7 (no. 7) are given.