Fig. 2.
Fig. 2. PS-341 induces JNK activation and HSP gene expression. / (A) PS-341 induces the phosphorylation of JNK, upstream molecule SEK1, and downstream targets c-Jun and ATF-2, in MM.1S cells in a time-dependent fashion. Immunoblotting with anti–α-tubulin antibody confirms equal protein loading. (B) PS-341 induces the phosphorylation of JNK and c-Jun in dose-dependent fashion. (C) PS-341 induces the phosphorylation of JNK in MM.1S, U266, and RPMI 8226 MM cell lines. (D) Gene microarray analysis demonstrates the up-regulation of HSP27 (♦), HSP40 (▪), HSP70 (▴), HSP90 (●), and HSP110 (○) mRNA in MM.1S cells treated with PS-341.

PS-341 induces JNK activation and HSP gene expression.

(A) PS-341 induces the phosphorylation of JNK, upstream molecule SEK1, and downstream targets c-Jun and ATF-2, in MM.1S cells in a time-dependent fashion. Immunoblotting with anti–α-tubulin antibody confirms equal protein loading. (B) PS-341 induces the phosphorylation of JNK and c-Jun in dose-dependent fashion. (C) PS-341 induces the phosphorylation of JNK in MM.1S, U266, and RPMI 8226 MM cell lines. (D) Gene microarray analysis demonstrates the up-regulation of HSP27 (♦), HSP40 (▪), HSP70 (▴), HSP90 (●), and HSP110 (○) mRNA in MM.1S cells treated with PS-341.

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