Microarray analysis of ITD transfectants reveals multiple differentially regulated genes.
Significance analysis of the microarray data (SAM analysis) between one group containing 4 microarrays of Flt3-WT with or without Flt3 ligand and another group containing 5 microarrays of Flt3-ITD with or without Flt3 ligand. The scatter plot of the observed difference(d(I)) versus the expected relative difference(dE(I)) is shown. The broken lines are drawn at a distance of Δ = 0.77 from the solid line that indicatesd(I) = dE(I). Genes outside the broken lines are regarded as genes with significant changes in expression, yielding 767 genes. The median estimated false discovery rate is 20%. Dots above the broken line indicate genes induced by Flt3-ITD (470), and dots below the broken line indicate genes that are suppressed by the ITD mutations (297).
