Fig. 2.
VEGF concentration in supernatants from unilineage cultures.
(A) Enzyme-linked immunosorbent assay performed at the indicated days in supernatant from HPCs differentiating through Mk, E, G, or Mo lineages. (B) VEGF concentration evaluated in supernatants from Mk cells cultured in the presence of 1, 5, or 100 ng/mL TPO (mean ± SEM from 3 independent experiments). (C) Effect of oxygen tension on VEGF release and ploidy: HPCs induced to unilineage Mk differentiation at either 5% or 20% O2 tension were analyzed for VEGF release (top panel) and ploidy (bottom panel); an aliquot of day 7 megakaryocytes from both culture conditions was switched to 1% O2 (middle panel). A representative experiment of 3 is shown.
